The Dhakuria flyover will be shut from 10pm on Friday to 6am on Monday for a load test, police said.
Built in 1963, the 380m-long bridge without piers has been under the scanner since the collapse of the Majerhat flyover in 2018 because of its crumbling structure.
The load test will be carried out to prepare a detailed project report for repairs and retrofitting works on the flyover, an official of the Calcutta Metropolitan Development Authority, the custodian of the flyover, said on Thursday.
Buses and minibuses headed towards the flyover from Ballygunge Phari will be diverted from the Gariahat crossing to Rashbehari Avenue, SP Mukherjee Road and Prince Anwar Shah Road. Buses moving towards the Bypass from near Jadavpur police station will be diverted from the Gariahat crossing towards Bijon Setu and further east towards Ruby.